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Micro-fluidic-based flow cytometer for point-of-care applications based on a spatial modulation technique
Conferences & Talks

BIOS 2009 (part of SPIE Photonics West) -- Microfluidics, BioMEMS, and Medical Microsystems

24 January 2009 - 29 January 2009
San Jose, California, USA

 

description

While commercial flow cytometers are sophisticated analytical instruments extensively used in research and clinical laboratories, they do not meet the challenging practical requirements for point-of-care (POC) diagnostics in resource-limited applications, such as CD4 monitoring in rural Africa. In this presentation we will describe and illustrate a fundamentally new design of the optical detection system which ultimately will enable a fluidic-chip-based flow cytometer that satisfies POC specifications for performance, robustness, compactness, cost, reagent consumption, and ease of use. The technology will be demonstrated with characterization of individual fluorescent beads (diameters: 6um, 2um and 0.6um), detection of tagged CD4 cells, and CD4 counting in whole blood.

 

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