Monitoring CD4 in whole blood with an opto-fluidic detector based on spatially modulated fluorescence emission
An optical detection technique termed “spatially modulated fluorescence emission” is described that delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use. The detection technique is demonstrated with measurements of absolute CD4+ and percentage CD4 counts in human blood. The performance is benchmarked by direct one-to-one comparison of measurements on the same tagged blood samples against a commercial instrument (BD FACSCount), with excellent agreement for both absolute CD4 and percentage CD4.
Kiesel, P.; Beck, M.; Johnson, N. M. Monitoring CD4 in whole blood with an opto-fluidic detector based on spatially modulated fluorescence emission. Cytometry Part A. 2011 April; A79 (4): 317-324.