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Quantitative analysis of protein-RNA interactions by gel mobility shift

 

The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to rsolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to elucidate thermodynamic properties of protein-RNA complexes. Assay designs for titration, competition and stoichiometry experiments are presented for two unrelated model complexes.

 
citation

Ryder, S. P.; Recht, M. I.; Williamson, J. R. Quantitative analysis of protein-RNA interactions by gel mobility shift. Methods in Molecular Biology. 2008; 488: 99-115.