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Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry

 

Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protein interactions as it provides the only method by which the thermodynamic parmeters of free energy, enthalpy and entropy can be directly determined. his chapter presents a general procedure for studying RNA-protein interactions using ITC.

 
citation

Recht, M. I.; Ryder, S. P.; Williamson, J. R. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry. Methods in Molecular Biology. 2008; 488: 117-127.