Flow cytometry on a chip
No commercial flow cytometer instrument meets all technical requirements for point-of-care detection in resource-limited settings; in particular the cost target remains extremely challenging. There are many interesting concepts under development especially for microfluidic-based flow cytometer. After a brief review of some new technologies important for on the flow anayte characterization we will describe an optical detection technique termed “spatially modulated fluorescence emission” developed at PARC which can delivers high signal-to-noise discrimination without precision optics to enable a flow cytometer that can combine high performance, robustness, compactness, low cost, and ease of use.
Kiesel, P.; Martini, J.; Recht, M. I.; Bern, M. W.; Johnson, N. M.; Huck, M. Flow Cytometry on a Chip. Chapter in Point of Care Diagnostics on a Chip, edited by D. Issadore and R. Westervelt. (Biological and Medical Physics, Biomedical Engineering) Berlin: Springer; 2013; 47-69.