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Electronic Readout ELISA with Organic Field-Effect Transistors as a Prognostic Test for Preeclampsia

 
The advent of point-of-care diagnostics has created a need for portable and real-time diagnostic tools. Application of organic field-effect transistors as sensors for clinically relevant analytes represents a viable approach in meeting this need. Here we demonstrate an electronic enzyme-linked immunosorbent readout assay for the detection of a panel of three distinct biomarkers, comprised of soluble fms-like tyrosine kinase, soluble endoglin, and leptin, to create a predictive indicator test for preeclampsia, a pregnancy syndrome resulting in severe and even fatal complications. Importantly, our sensors can be operated in both undiluted buffer and fetal bovine serum. To underscore our ability to produce inexpensive and even disposable devices using large-area fabrication techniques, we demonstrated biodetection utilizing a fully inkjet-printed and flexible organic field-effect transistor.
 
citation

Hammock, M.; Knopfmacher, O.; Ng, T.; Tok, J. B.; Bao, Z. Electronic Readout ELISA with Organic Field-Effect Transistors as a Prognostic Test for Preeclampsia. Advanced Materials, 26: 6138–6144. doi: 10.1002/adma.201401829