Imaging Diagnostics: From Microspheres To Live Cell Assays

The first half of the talk will focus on the development of particle imaging techniques and its application to microchannel flows. Particle image velocimetry and particle tracking velocimetry measurements in pressure and electrokinetically-driven flows are presented with a quantitative demonstration of similarity between electric and velocity fields. Application of these particle imaging techniques to gain new insight into the dynamics of field amplified sample stacking and particle fractionation in microfluidic systems is shown.

The second half of the talk will illustrate the application of wide-field total internal reflection fluorescence imaging to study protein translocation within live cells. Surface patterning techniques were applied to Cytop-coated slides to form cell arrays. Stimuli for the activation and translocation of signaling proteins were delivered by a custom-built automated perfusion system. Full field temporally-resolved measurements of protein translocation events to the plasma membrane in cell populations with single cell resolution are presented.