Micro-fluidic-based flow cytometer for point-of-care applications based on a spatial modulation technique
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Micro-fluidic-based flow cytometer for point-of-care applications based on a spatial modulation technique
While commercial flow cytometers are sophisticated analytical instruments extensively used in research and clinical laboratories, they do not meet the challenging practical requirements for point-of-care (POC) diagnostics in resource-limited applications, such as CD4 monitoring in rural Africa. In this presentation we will describe and illustrate a fundamentally new design of the optical detection system which ultimately will enable a fluidic-chip-based flow cytometer that satisfies POC specifications for performance, robustness, compactness, cost, reagent consumption, and ease of use. The technology will be demonstrated with characterization of individual fluorescent beads (diameters: 6um, 2um and 0.6um), detection of tagged CD4 cells, and CD4 counting in whole blood.
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