Miniaturized opto-fluidic system for on-the-flow analyte characterization based on spatial modulation technique

Details

Event Proceedings for 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Authors

Recht, Michael I.
Marshall Bern
Noble Johnson
Technical Publications
October 27th 2013
A compact opto-fluidic detection platform for on-the-flow analyte characterization is described. The performance is demonstrated with recent results for water-borne pathogens. Results are obtained for Giardia, Cryptosporidium, and E. Coli, with reliable identification and enumeration of the pathogens at meaningfully low concentrations. Incubation studies with antibody-based reagents show that for Giardia incubation times as short as 2 min and analyte-to-reagent ratios as low as 1:100 are sufficient for reliable detection. And the antibody-based reagents (in liquid form) are highly stable, with little degradation over a period of months at 37 degrees C.

Citation

Kiesel, P.; Martini, J.; Recht, M. I.; Bern, M. W.; Johnson, N. M. Miniaturized opto-fluidic system for on-the-flow analyte characterization based on spatial modulation technique. Proceedings of 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2013); 2013 October 27-31; Freiburg, Germany.

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