Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry

Details

Event Methods in Molecular Biology

Authors

Recht, Michael I.
Technical Publications
December 31st 2008
Isothermal titration calorimetry (ITC) is a useful technique to study RNA-protein interactions as it provides the only method by which the thermodynamic parmeters of free energy, enthalpy and entropy can be directly determined. his chapter presents a general procedure for studying RNA-protein interactions using ITC.

Citation

Recht, M. I.; Ryder, S. P.; Williamson, J. R. Monitoring assembly of ribonucleoprotein complexes by isothermal titration calorimetry. Methods in Molecular Biology. 2008; 488: 117-127.

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