Quantitative analysis of protein-RNA interactions by gel mobility shift

Details

Event Methods in Molecular Biology

Authors

Recht, Michael I.
Technical Publications
December 31st 2008
The gel mobility shift assay is routinely used to visualize protein-RNA interactions. Its power resides in the ability to rsolve free from bound RNA with high resolution in a gel matrix. We review the quantitative application of this approach to elucidate thermodynamic properties of protein-RNA complexes. Assay designs for titration, competition and stoichiometry experiments are presented for two unrelated model complexes.

Citation

Ryder, S. P.; Recht, M. I.; Williamson, J. R. Quantitative analysis of protein-RNA interactions by gel mobility shift. Methods in Molecular Biology. 2008; 488: 99-115.

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