Rapid mixing of sub-microliter drops by magnetic micro-stirring

Details

Event Lab on a Chip

Authors

Dirk De Bruyker
Recht, Michael I.
Alan Bell
Bruce, Richard H.
Technical Publications
August 15th 2011
We demonstrate rapid mixing of sub-microliter droplets (250nl) using miniaturized magnetic stir bars (400 m by 200 m by 15 m). The stir bars are fabricated using laser micromachining and placed on the substrate on which the drops are manipulated. They are activated by an externally applied magnetic field and used in combination with on-demand drop merging in enthalpy arrays. This technique results in a 10 fold increase in mixing rate, and a mixing time constant of about 2 seconds. Drop mixing times are measured by Frster resonance energy transfer (FRET) and verified by thermodynamic measurements of binding and enzymatic reactions.

Citation

De Bruyker, D.; Recht, M. I.; Bhagat, A.; Torres, F.; Bell, A. G.; Bruce, R. H. Rapid mixing of sub-microliter drops by magnetic micro-stirring. Lab on a Chip. 2011 October 7; 11 (19): 3313-3319.

Additional information

Focus Areas

Our work is centered around a series of Focus Areas that we believe are the future of science and technology.

FIND OUT MORE
Licensing & Commercialization Opportunities

We’re continually developing new technologies, many of which are available for¬†Commercialization.

FIND OUT MORE
News

PARC scientists and staffers are active members and contributors to the science and technology communities.

FIND OUT MORE